Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment

XTBG OpenIR > 能源植物分子育种研究组

	Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment
	Mao-Sheng Chen; Bang-Zhen Pan; Gui-Juan Wang; Jun Ni; Longjian Niu; Zeng-Fu Xu
	2014
Source Publication	BMC Plant Biology
Volume	14 Issue:318
Abstract	Background Jatropha curcas L. is a potential biofuel plant. Application of exogenous cytokinin (6-benzyladenine, BA) on its inflorescence buds can significantly increase the number of female flowers, thereby improving seed yield. To investigate which genes and signal pathways are involved in the response to cytokinin in J. curcasinflorescence buds, we monitored transcriptional activity in inflorescences at 0, 3, 12, 24, and 48 h after BA treatment using a microarray. Results We detected 5,555 differentially expressed transcripts over the course of the experiment, which could be grouped into 12 distinct temporal expression patterns. We also identified 31 and 131 transcripts in J. curcaswhose homologs in model plants function in flowering and phytohormonal signaling pathways, respectively. According to the transcriptional analysis of genes involved in flower development, we hypothesized that BA treatment delays floral organ formation by inhibiting the transcription of the A, B and E classes of floral organ-identity genes, which would allow more time to generate more floral primordia in inflorescence meristems, thereby enhancing inflorescence branching and significantly increasing flower number per inflorescence. BA treatment might also play an important role in maintaining the flowering signals by activating the transcription of GIGANTEA (GI) and inactivating the transcription of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and TERMINAL FLOWER 1b (TFL1b). In addition, exogenous cytokinin treatment could regulate the expression of genes involved in the metabolism and signaling of other phytohormones, indicating that cytokinin and other phytohormones jointly regulate flower development inJ. curcas inflorescence buds. Conclusions Our study provides a framework to better understand the molecular mechanisms underlying changes in flowering traits in response to cytokinin treatment in J. curcas inflorescence buds. The results provide valuable information related to the mechanisms of cross-talk among multiple phytohormone signaling pathways in woody plants.
Document Type	期刊论文
Identifier	https://ir.xtbg.ac.cn/handle/353005/10078
Collection	能源植物分子育种研究组
Recommended Citation GB/T 7714	Mao-Sheng Chen,Bang-Zhen Pan,Gui-Juan Wang,et al. Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment[J]. BMC Plant Biology,2014,14(318).
APA	Mao-Sheng Chen,Bang-Zhen Pan,Gui-Juan Wang,Jun Ni,Longjian Niu,&Zeng-Fu Xu.(2014).Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment.BMC Plant Biology,14(318).
MLA	Mao-Sheng Chen,et al."Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment".BMC Plant Biology 14.318(2014).

Files in This Item:		Download All
File Name/Size	DocType	Version	Access	License
Analysis of the tran（2699KB）			开放获取	CC BY-NC-SA	View Download

File name:	Analysis of the transcriptional responses in inflorescence buds of Jatropha curcasexposed to cytokinin treatment.pdf
Format:	Adobe PDF

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

能源植物分子育种研究组知识库

Background

Results

Conclusions